Results 1–3 of 3 for spektrofotometar
Spectrophotometer is an instrument for measuring the amount of light absorbed by a sample.
The absorption of light by a substance in a solution can be described mathematically by the Beer-Lambert law
where A is the absorbance at a given wavelength of light, ε is the molar absorbtivity or extinction coefficient (L mol-1 cm-1), unique to each molecule and varying with wavelength, b is the length of light path through the sample (cm), and c is the concentration of the compound in solution (mol L-1).
Absorbance (A) is a logarithm of the ratio of incident radiant power (Po) to transmitted radiant power (P) through a sample (excluding the effects on cell walls).
The absorption of light by a substance in a solution can be described mathematically by the Beer-Lambert law
where A is the absorbance at a given wavelength of light, ε is the molar absorbtivity or extinction coefficient (L mol-1 cm-1), unique to each molecule and varying with wavelength, b is the length of light path through the sample (cm), and c is the concentration of the compound in solution (mol L-1).
Beer’s law (or Beer-Lambert law) is the functional relationship between the quantity measured in an absorption method (A) and the quantity sought, the analyte concentration (c). As a consequence of interactions between the photons and absorbing particles, the power of the beam is attenuated from Po to P. Beer’s law can be written
where A is the absorbance at a given wavelength of light, ε is the molar absorbtivity or extinction coefficient (L mol-1 cm-1), unique to each molecule and varying with wavelength, b is the length of light path through the sample (cm), and c is the concentration of the compound in solution (mol L-1).
Generalic, Eni. "Spektrofotometar." Croatian-English Chemistry Dictionary & Glossary. 29 June 2022. KTF-Split. 22 Apr. 2025. <https://glossary.periodni.com>.
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