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Electrophoresis is a technique for the analysis and separation of colloids, based on the movement of charged colloidal particles in an electric field. The migration is toward electrodes of charge opposite to that of the particles. The rate of migration of the particles depends on the field, the charge on the particles, and on other factors, such as the size and shape of the particles.
Electrophoresis is important in the study of proteins. The acidity of the solution can be used to control the direction in which a protein moves upon electrophoresis.
Colloid ions emerge when colloid particles adsorb certain type of ion from solution and thus become charged with the same charge. The charge can also originate form a chemical reaction of colloid particle’s surface. Colloid ions formed by absorption of silver chloride particle can be show as follows:
Adsorbed layer is monomolecular (one molecule thick) and which type of ion will be formed depends upon which ions are present in a greater number in the solution in. Because of this colloid particles are charged with the same charge, mutual repelling occurs, and the colloid solution becomes stable. Colloid charge can be determined by electrophoresis.
Generalic, Eni. "Elektroforeza." Croatian-English Chemistry Dictionary & Glossary. 29 June 2022. KTF-Split. 21 Apr. 2025. <https://glossary.periodni.com>.
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