Referent electrode is an electrode whose potential is known and completely independent of analyte concentration. Mostly used referent electrodes are calomel and silver/silver chloride electrode.
Table: Dependence of referent electrodes potentials on KCl concentration
| Potential vs. SHE / V | |||||
| calomel electrode | Ag/AgCl electrode | ||||
| t / °C | 0.1 mol dm-3 | 3.5 mol dm-3 | sat. solution | 3.5 mol dm-3 | sat. solution |
| 15 | 0.3362 | 0.254 | 0.2511 | 0.212 | 0.209 |
| 20 | 0.3359 | 0.252 | 0.2479 | 0.208 | 0.204 |
| 25 | 0.3356 | 0.250 | 0.2444 | 0.205 | 0.199 |
| 30 | 0.3351 | 0.248 | 0.2411 | 0.201 | 0.194 |
| 35 | 0.3344 | 0.246 | 0.2376 | 0.197 | 0.189 |
Human eye can only see electromagnetic radiation of wavelengths form 400 nm to 760 nm. This narrow part of electromagnetic spectrum is called visible radiation. Visible (white) light is a mixture of light of all kind of colours, it can be separated, with the help of a glass prism, into its component colours - visible light spectrum, and each colour corresponds to a certain area of wavelengths:
| Colour | Wavelength / nm |
|---|---|
| purple | 400 - 450 |
| blue | 450 - 500 |
| green | 500 - 570 |
| yellow | 570 - 590 |
| orange | 590 - 620 |
| red | 620 - 760 |
Volumetric flasks are bottles made of glass, in a pear like in shape with long thin necks and flat bottoms. All come with a ground glass stopper for a tight seal. Volume marking is cut in glass with fluoride acid around the neck, so that parallax should be avoided (flask is put in front of the eyes so that one can see only a straight horizontal line). A volumetric flask is calibrated to contain (TC or In) the indicated volume of water at 20 °C when the bottom of the meniscus is adjusted to just rest on the center of the line marked on the neck of the flask. They are used for preparing the exactly known volume of sample solution and standard solutions of reagents. On each flask with volume designation a temperature on which the flask has been calibrated is designated.
Generalic, Eni. "ARE CENTER." Croatian-English Chemistry Dictionary & Glossary. 29 June 2022. KTF-Split. {Date of access}. <https://glossary.periodni.com>.
Glossary
Periodic Table
